Antibacterial activity on electrospun poly(lactide-co-glycolide) based membranes via Magainin II grafting

• PLGA and PLGA/gelatin fibrous membranes were prepared by electrospinning.
• Antimicrobial peptide Mag II was successfully immobilized on PLGA based membranes.
• The antibacterial activity was tested against E. coli and S. aureus.
• Bacterial adhesion was inhibited on Mag II functionalized membranes.

An antimicrobial peptide (AMP), Magainin II (Mag II) was covalently immobilized on poly(lactide-co-glycolide) (PLGA) and PLGA/gelatin electrospun fibrous membranes. The surface immobilization was characterized by X-ray Photoelectron Spectroscopy (XPS). Scanning Electron Microscopy (SEM) and Atomic Force Microscopy studies showed that the surface morphology of the fibers at micron scale was not affected by the immobilization process. The antibacterial activity of the bound Mag II was tested against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. Bacterial adhesion tests, SEM and confocal analyses revealed that the attachment and survival of bacteria were inhibited on Mag II functionalized membranes. AMP immobilization strategy was introduced as a new perspective for the modulation of antibacterial properties on PLGA based materials prepared by electrospinning.