Application of enzyme/zeolite sensor for urea analysis in serum

• The enzyme/zeolite sensor was created by urease adsorption on silicalite.
• The sensor is notable for its simple and fast performance and high reproducibility.
• Urea content was successfully measured in samples of blood serum.
• Accuracy of urea determination was 10%; total time of analysis was 10 min.
• Biosensor results correlated well with the traditional method of urea determination.

Urea biosensor based on zeolite-adsorbed urease was applied for analysis of blood serum samples. It should be noted, that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, high reproducibility and repeatability (RSD = 9% and 4%, respectively). The linear range of urea determination by using the biosensor was 0.003–0.75 mM, and the limit of urea detection was 3 μM. The method of standard addition was used for analysis of serum samples with 500-fold dilution. Total time of analysis was 10 min. Good reproducibility of urea determination in real samples was demonstrated (RSD = 10%). Biosensor results were verified by using a common method of urea determination (diacetyl monoxime reaction). It was shown that by using this biosensor distinguishing healthy people from people with renal dysfunction becomes easier.

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