Glucose-sensitive colorimetric sensor based on peroxidase mimics activity of porphyrin-Fe3O4 nanocomposites

• H2TCPP-Fe3O4 nanocomposites exhibite ultra-high peroxidase-like activity.
• A novel colorimetric sensor for glucose with a lower detection limit is provided.
• The catalase-mimic activity of the H2TCPP-Fe3O4 nanocomposites originates from OH · .
• H2TCPP-Fe3O4 nanocomposites show high sensitivity, selectivity and high stability.

5,10,15,20-Tetrakis(4-carboxyphenyl)-porphyrin-functionalized Fe3O4 nanocomposites (H2TCPP-Fe3O4) were successfully prepared by a simple two-step method. These nanocomposites exhibited ultra-high peroxidase-like activity compared with pure Fe3O4 nanoparticles. Colorless peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) was changed by H2O2 to its blue oxidized state. Kinetic studies indicated that the H2TCPP-Fe3O4 nanocomposites exhibited enhanced affinity toward H2O2 with a higher catalytic activity than Fe3O4 nanoparticles alone. Results of a fluorescent probe suggested that the catalase-mimic activity of the H2TCPP-Fe3O4 nanocomposites effectively catalyzed the decomposition of H2O2 into hydroxyl radicals. A simple, sensitive, and selective visual and colorimetric method with TMB as the substrate was designed to detect glucose when combined with glucose oxidase. This colorimetric method can be used for colorimetric detection of H2O2 with a minimum detection limit of 1.07 × 10− 6 M and a dynamic range of 5 × 10− 6 mol · L− 1 to 8 × 10− 5 mol · L− 1. This method can also be used to detect glucose at a minimum detection limit of 2.21 × 10− 6 M and a dynamic range of 25 × 10− 6 mol · L− 1 to 5 × 10− 6 mol · L− 1. Furthermore, the robustness of the nanocomposites makes them suitable for a wide range of applications in biomedicine and environmental chemistry fields.

5,10,15,20-tetrakis(4-carboxyl phenyl)-porphyrin (H2TCPP)-Fe3O4 nanocomposites were demonstrated to possess intrinsic peroxidase-like activity and a higher catalytic activity, compared to that of Fe3O4 NPs alone.Figure optionsDownload as PowerPoint slide