کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428952 987161 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly stable, protein capped gold nanoparticles as effective drug delivery vehicles for amino-glycosidic antibiotics
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Highly stable, protein capped gold nanoparticles as effective drug delivery vehicles for amino-glycosidic antibiotics
چکیده انگلیسی

A method for the production of highly stable gold nanoparticles (Au NP) was optimized using sodium borohydride as reducing agent and bovine serum albumin as capping agent. The synthesized nanoparticles were characterized using UV–visible spectroscopy, transmission electron microscopy, X‐ray diffraction (XRD) and dynamic light scattering techniques. The formation of gold nanoparticles was confirmed from the appearance of pink colour and an absorption maximum at 532 nm. These protein capped nanoparticles exhibited excellent stability towards pH modification and electrolyte addition. The produced nanoparticles were found to be spherical in shape, nearly monodispersed and with an average particle size of 7.8 ± 1.7 nm. Crystalline nature of the nanoparticles in face centered cubic structure is confirmed from the selected‐area electron diffraction and XRD patterns. The nanoparticles were functionalized with various amino-glycosidic antibiotics for utilizing them as drug delivery vehicles. Using Fourier transform infrared spectroscopy, the possible functional groups of antibiotics bound to the nanoparticle surface have been examined. These drug loaded nanoparticle solutions were tested for their antibacterial activity against Gram-negative and Gram-positive bacterial strains, by well diffusion assay. The antibiotic conjugated Au NP exhibited enhanced antibacterial activity, compared to pure antibiotic at the same concentration. Being protein capped and highly stable, these gold nanoparticles can act as effective carriers for drugs and might have considerable applications in the field of infection prevention and therapeutics.


► Method for NaBH4 reduced and BSA capped gold nanoparticle was standardized.
► Nanoparticles were spherical and nearly monodispersed with a size of 7.8 nm.
► Nanoparticles are extremely stable towards pH modification and electrolyte addition.
► Antibiotic conjugated nanoparticles exhibited enhanced antibacterial activity.
► Protein capped and highly stable nanoparticles can act as effective drug carriers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 32, Issue 6, 1 August 2012, Pages 1571–1577
نویسندگان
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