کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429165 987165 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biosensor based on laccase immobilized on plasma polymerized allylamine/carbon electrode
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Biosensor based on laccase immobilized on plasma polymerized allylamine/carbon electrode
چکیده انگلیسی


• Low pressure plasma was used to generate stable allylamine coating.
• Laccase from Trametes versicolor was physisorbed and covalently immobilized.
• Best biosensor efficiency obtained for the covalently immobilized laccases
• Sensitivities of 4.8 μA mg− 1 L and 2.7 μA mg− 1 L for ABTS and DMP respectively

In this work, a simple and rapid method was used to functionalize carbon electrode in order to efficiently immobilize laccase for biosensor application. A stable allylamine coating was deposited using a low pressure inductively excited RF tubular plasma reactor under mild plasma conditions (low plasma power (10 W), few minutes) to generate high density amine groups (N/C ratio up to 0.18) on rough carbon surface electrodes. The longer was the allylamine plasma deposition time; the better was the surface coverage. Laccase from Trametes versicolor was physisorbed and covalently bound to these allylamine modified carbon surfaces. The laccase activities and current outputs measured in the presence of 2,2′-azinobis-(3-ethylbenzothiazole-6-sulfonic acid) (ABTS) showed that the best efficiency was obtained for electrode plasma coated during 30 min. They showed also that for all the tested electrodes, the activities and current outputs of the covalently immobilized laccases were twice higher than the physically adsorbed ones. The sensitivity of these biocompatible bioelectrodes was evaluated by measuring their catalytic efficiency for oxygen reduction in the presence of ABTS as non-phenolic redox substrate and 2,6-dimethoxyphenol (DMP) as phenolic one. Sensitivities of around 4.8 μA mg− 1 L and 2.7 μA mg− 1 L were attained for ABTS and DMP respectively. An excellent stability of this laccase biosensor was observed for over 6 months.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 33, Issue 6, 1 August 2013, Pages 3197–3205
نویسندگان
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