Electrochemical oxidation of adenosine-5′-triphosphate on a chitosan–graphene composite modified carbon ionic liquid electrode and its determination

In this paper a new electrochemical method was proposed for the determination of adenosine-5′-triphosphate (ATP) based on a chitosan (CTS) and graphene (GR) composite film modified carbon ionic liquid electrode (CTS–GR/CILE). CILE was fabricated by using ionic liquid 1-butyl-3-methylimidazolium dihydrogen phosphate ([BMIM]H2PO4) as the binder, which was further modified by GR and CTS composite. The modified electrode exhibited an excellent electrocatalytic activity toward the oxidation of ATP with the increase of the oxidation peak current and the decrease of the oxidation peak potential. The electrochemical parameters of ATP on CTS–GR/CILE were calculated with the electron transfer coefficient (α) as 0.329, the electron transfer number (n) as 2.15, the apparent heterogeneous electron transfer rate constant (ks) as 3.705 × 10− 5 s− 1 and the surface coverage (ΓT) as 9.33 × 10− 10 mol cm− 2. Under the optimal conditions the oxidation peak current was proportional to ATP concentration in the range from 1.0 × 10− 6 to 1.0 × 10− 3 M with the detection limit of 0.311 μM (S/N = 3). The proposed electrode showed excellent reproducibility, stability, anti-interference ability and further successfully applied to the ATP injection sample detection.

► Ionic liquid [BMIM]H2PO4 based carbon ionic liquid electrode (CILE) was prepared.
► Graphene modified CILE was fabricated for the sensitive electrochemical detection of ATP.
► Good electrocatalytic ability to the ATP oxidation was achieved.
► Detection of 5′-ATP in commercial injection samples with satisfactory results.