کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1429692 | 1509188 | 2010 | 6 صفحه PDF | دانلود رایگان |

One of the crucial steps in gene delivery with non-viral vectors is the escape of DNA complexes from the endosome. In order to improve gene transfection efficiency, we designed a novel gene delivery vector gelatin–siloxane nanoparticles (GS NPs) conjugated with two different membrane-destabilizing peptides, octaarginine (R8) and a subunit of influenza virus haemagglutinin HA2. Both R8-GS NPs and HA2-GS NPs had high positive surface charges. They could condense and protect DNA against serum/DNase degradation. Results from flow cytometry and confocal laser scanning microscope respectively indicated that R8-GS NPs had higher uptake efficiency than HA2-GS NPs, whereas HA2-GS had higher endosome escaping efficiency. Furthermore, in vitro transfection displayed a higher gene expression level with HA2-modified GS NPs, which suggested that endosome escaping is the crucial step for nanoparticle mediated gene therapy.
Journal: Materials Science and Engineering: C - Volume 30, Issue 8, 12 October 2010, Pages 1260–1265