کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1431092 1509189 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Under flow impedimetric measurements using magnetic particles for label-free detection affinity target
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Under flow impedimetric measurements using magnetic particles for label-free detection affinity target
چکیده انگلیسی

The necessity to adapt sensors based on electrochemical techniques for high throughput analysis control increases the interest to develop new analytical systems able to perform measurements under buffer flow.In this report we explored the possibility of employing a new system to make impedimetric measurements to detect the interaction between proteins and small molecules. The well-known biotin–streptavidin interaction was adopted to evaluate the proposed assembly. This system allows us to perform experiments under flow. Magnetic beads functionalized with streptavidin were used and first characterized using AFM and FTIR.Non-faradic impedance spectroscopy allowed the detection of the biotin–streptavidin interaction. Using our new system and under a flow of PBS buffer, 5 10− 5 M of biotin was detected with a stable signal.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 28, Issues 5–6, 1 July 2008, Pages 820–825
نویسندگان
, , , , , ,