Lipase immobilized by modification-coupled and adsorption–cross-linking methods: A comparative study

Candida antarctica lipase was immobilized by an adsorption and cross-linking method with NW-ZT2 and by modification-coupled method with a silica–PEG gel. The final product silica–PEG-lipase was confirmed by IR spectra. The optimum pH value, the optimum temperature, the thermo-stabilities and operational stabilities for two kinds of immobilized lipase were also determined. Results show that the silica–PEG-lipase gel was superior to the lipase immobilized by adsorption and cross-linking, however both are viable for use in transesterification reactions.