کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
14743 | 1245 | 2006 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Advances and applications of de novo designed affinity ligands in proteomics Advances and applications of de novo designed affinity ligands in proteomics](/preview/png/14743.png)
Affinity chromatography represents a promising technique for decoding the proteomics universe. While conventional affinity purification is being used in conjunction with two-dimensional electrophoresis (2D-PAGE) and mass spectrometry (MS) for the study of proteomes and subproteomes, scientists are still confronted with the need for specific and tailor-made affinity ligands to target desired groups and families of proteins. Evidence has shown that, in many situations, synthetic affinity ligands can circumvent inconveniences associated with the utilisation of biological ligands for the chromatography-based purification of biomolecules. This review will highlight the potential applications of affinity chromatography and synthetic de novo designed ligands as separation tools for proteomics.
Journal: Biotechnology Advances - Volume 24, Issue 1, January–February 2006, Pages 17–26