کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1482166 | 991555 | 2011 | 7 صفحه PDF | دانلود رایگان |
The main aim of the present work is to investigate the dynamical effects of the addition of disaccharides to hydrated lysozyme. The Self-Distribution-Function procedure is at first applied on Elastic Incoherent Neutron Scattering data obtained by the IN13 spectrometer on trehalose/H2O and sucrose/H2O where it highlights a different Q-dependence for the two disaccharides. Then a quantitative analysis of the Mean Square Displacement of lysozyme/trehalose/H2O and of lysozyme/sucrose/H2O from Elastic Incoherent Neutron Scattering data obtained by the IN10 spectrometer is presented. It is shown how the resolution function gives rise to a time integration of a given time-dependent Mean Square Displacement function. Furthermore the analysis shows that the protein dynamical transition, registered at a temperature value of about T = 200–220 K in the H2O hydrated lysozyme sample, is inhibited by the addition of the disaccharides.
Journal: Journal of Non-Crystalline Solids - Volume 357, Issue 2, 15 January 2011, Pages 664–670