کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
148476 456416 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The spinning cloth disc reactor for immobilized enzymes: A new process intensification technology for enzymatic reactions
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
The spinning cloth disc reactor for immobilized enzymes: A new process intensification technology for enzymatic reactions
چکیده انگلیسی

The spinning cloth disc reactor (SCDR) is an innovative enzyme reaction intensification technology. Based on spinning disc technology, the SCDR uses centrifugal forces to allow an even spread of a thin film across a spinning horizontal disc which holds a cloth with immobilized enzyme. This geometry promotes accelerated reactions due to high mass transfer rates and rapid mixing. Here, the SCDR has been benchmarked against a conventional batch stirred tank reactor (BSTR) using tributyrin emulsion hydrolysis as a model reaction and lipase immobilized on woolen cloth as the biocatalyst. Reaction intensification has been shown to occur: the conversion in the SCDR was significantly higher than that in a conventional BSTR under comparable conditions. Spinning speed and flow rate control reaction rate and conversion: conversion increased nearly 7% on average as the flow rate rose from 2 to 5 mL s−1 and the highest conversion (72.1%) occurred at 400 rpm. A Ping Pong Bi Bi kinetic model fitted reaction progress data well. The immobilized lipase showed excellent stability to repeat reactions in the SCDR: 80% of the original activity was retained after 15 consecutive runs. The robustness of the SCDR to industrially relevant feeds was also demonstrated through successful hydrolysis of different vegetable oils at reaction rates 5 times higher than other reactors in the literature. Overall, the above results indicate that the SCDR is an innovative, superior and robust technology for enhancing enzyme reactions, taking enzyme reactors beyond the current state-of-the-art. This concept can readily be extended to other enzyme-catalyzed reactions, where enhanced mass transfer and enzyme stability is needed.


► A novel rotating enzyme reactor was developed: spinning cloth disc reactor (SCDR).
► Process intensification occurred in SCDR: conversion and rate were largely improvfed.
► Below an average surface shear of 9500 s−1 enzyme loss in SCDR was very slight.
► Immobilized enzyme retained 80% initial activity after 15 long term reuses in SCDR.
► A Ping Pong Bi Bi kinetic model fitted well with the experimental data.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Journal - Volume 221, 1 April 2013, Pages 407–417
نویسندگان
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