A new supercritical assisted atomization configuration, for the micronization of thermolabile compounds

A novel process configuration of supercritical assisted atomization (SAA) was used to produce microparticles of two model thermolabile compounds: poly l-lactide (PLLA) and bovine serum albumin (BSA). This new configuration allowed to operate the precipitation process below the atmospheric pressure, to perform the drying process at lower temperatures without any damage to thermolabile materials. Process parameters, such as gas to liquid ratio and precipitation temperature, were investigated to evaluate their influence on particle size and morphology. Well defined spherical particles were produced. PLLA microparticles coalescence was avoided using a precipitation pressure from 0.65 to 0.5 bar producing particles with a mean diameter between 1 and 1.5 μm, and a standard deviation of about 0.4. BSA spherical particles with a mean diameter of about 1 μm, and a standard deviation of about 0.7, were obtained operating at 0.8 bar in the precipitator. Fourier transform infrared analysis on BSA microparticles revealed that no modification on protein secondary structure took place during SAA process, thus, protein denaturation was avoided.

► Thermolabile materials, Poly(l-lactide) and bovine serum albumin, were micronized using Supercritical Assisted Atomization.
► A vacuum system was added to the traditional configuration to allow the reduction of the precipitation temperature.
► The obtained particles have a regular morphology.
► No modification of the structure were revealed.
► The obtained particles are suitable for lung administration.