کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
153066 456518 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enrichment of n-3 polyunsaturated fatty acids into acylglycerols of borage oil via lipase-catalyzed reactions under supercritical conditions
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Enrichment of n-3 polyunsaturated fatty acids into acylglycerols of borage oil via lipase-catalyzed reactions under supercritical conditions
چکیده انگلیسی

Esterification of free n-3 polyunsaturated fatty acid (PUFA) with enriched n-6 triglyceride (TG) was catalyzed to modify the desired structured lipid with n-3/n-6 = 4 by using lipase under supercritical carbon dioxide (SCCO2). Comparing four different types of lipases, 1,3-specific lipase from Mucor miehei has the highest degree of incorporation under 10 wt.% loading amount of total substrates. The optimal operating parameters under 10.2 MPa and 323.15 K SCCO2 can attain the desired n-3/n-6 ratio in 6 h. Because of the negative effect on the enzyme activity by the enriched n-6 TG, the optimal substrate ratio of the enriched n-6 TG and the n-3 PUFA was chosen as 1/4. To enhance the solubility of n-3 PUFA in SCCO2, ethanol was applied as a co-solvent and reached an optimal input at 10 wt.% of total substrates. The activity of the enzyme still maintained 81% of initial activity because of de-moisture from the surface enzyme after seven cyclic pressurization/depressurizations. A model of the ping-pong mechanism and the substrate inhibition was proposed to express the catalytic action of lipase. With the aid of the Michaelis–Menten equation and Lineweaver–Burk plot, the estimated reaction rate has a deviation of only 2.11% from the experiment data.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Journal - Volume 141, Issues 1–3, 15 July 2008, Pages 318–326
نویسندگان
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