A study on the influence of ethanol over the primary cultured rat cortical neurons by using the scanning electron microscopy

As an inhibitor and toxic factor of central nervous system, ethanol inhibits the action of the neurons and causes various kinds of neuronal damage. However, the precise mechanisms that ethanol-induced neuronal damage in the central nervous system remain unclear. In spite of thousands of published studies, little information is available on the neurons’ morphological alteration in the central nervous system. In this study, we investigated the morphological alterations of the primary cultured rat cortical neurons after they were treated by different concentrations of ethanol using the scanning electron microscopy. Our results showed that the moderate or high concentration of ethanol could lead to morphological changes of these cultured rat cortical neurons, and they were closely associated with the duration of time. Our study will provide a new base for further studies on the effects of ethanol in the central nervous system.

► Ethanol is an inhibitor of central nervous system.
► The moderate or high concentration of ethanol could lead to the morphological changes of these cultured rat cortical neurons.
► The morphological alterations of primary cultured rat cortical neurons is closely associated with the concentrations of ethanol.
► The morphological alterations of primary cultured rat cortical neurons is also closely associated the duration time.