Characterization and evaluation of anti-biofilm effect of green synthesized copper nanoparticles

Copper nanoparticles (CuNPs) have been synthesized using two stage chemical reduction method, where a separate reducing agent Hydrazine Hydrate (HH) and a separate stabilizing agent Gum Kondagogu extract are used. The synthesized CuNPs are characterized by using Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), UV visible spectroscopy, X-Ray Diffraction (XRD), FT-Raman spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR) experimental methods. These experimental results reveal the formation of stable FCC crystalline CuNPs with thin Gum Kondagogu extract layer around their surface. Biofilm is the major target for the development of drugs and it is related to most preferred type of highly infectious factor of pathogenic microorganisms. A biofilm promotes persistence of bacteria by resisting antibiotic treatment. In the present study, anti-biofilm effect of Gum Kondagogu extract stabilized CuNPs against clinical isolate Klebsiella Pneumoniae (Gram -ve, ATCC 27736) bacteria is tested. SEM results reveal effective anti-biofilm effect on the CuNPs film. The present study would encourage the development of anti bacterial coated medical devices against pathogenic microorganisms.