Multiscale engineered hierarchical structures with precisely controlled sizes for bio-inspired cell culture

• Simple yet effective approach to fabricate multiscale ECM like structures were proposed.
• Precisely defined 250 nm patterns were fabricated on micro-sized wrinkling structure.
• Multiscale topographical cues on morphologies and orientations of cells were investigated.

We present a simple method of fabricating bio-inspired multiscale structure by combining replica molding and surface wrinkling. With this technique, we demonstrates that our fabrication method is manageable to fabricate various types of substrate with various orientations of nanopatterns on microwrinkle structure, resulting perfectly conform the wrinkling phenomenon. Finally we have explored cells behavior on multiscale substrate.