Application of hybrid mesoporous silica for extraction of hormones in milk by matrix solid phase dispersion

• A new analytical method for determination of steroids in milk was proposed.
• Hybrid SBA-15 was applied for the first time as solid support in MSPD.
• The method was rapid, simple and efficient for the determination of steroids by MECK.

A matrix solid phase dispersion (MSPD) method for simultaneous determination of five natural and synthetic steroids in goat milk was proposed. SBA-15 type mesoporous silica were functionalized with octadecylsilane, and the resulting hybrid material (SBA-15-C18) was applied as a solid support for MSPD. The synthesized material showed high affinity to steroids in milk, and the obtained extract was sufficiently cleaned to be directly analyzed by micellar electrokinetic chromatography (MECK). The proposed method was validated in terms of linearity, precision, accuracy, limit of detection and quantitation. To the best of our knowledge this is the first application where hybrid mesoporous silica have been used to determine hormones in milk by a MSPD–MECK method.