کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1648948 | 1007569 | 2009 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: On-chip encapsulation of lipase using mesoporous silica: A new route to enzyme microreactors On-chip encapsulation of lipase using mesoporous silica: A new route to enzyme microreactors](/preview/png/1648948.png)
A polydimethylsiloxane/glass microfluidic reactor containing lipase–mesoporous silica (FSM-22) conjugates has been successfully constructed without chemical cross-linking between enzyme and support. A direct visualization of conjugates of lipase and FSM-22 immobilized in the microreactor by optical microscopy revealed that the enzymes were uniformly dispersed throughout the particles of the FSM-22, because of the successful immobilization of the enzyme. Moreover, the lipase–FSM-22 conjugates contained in the microreactor indicated higher enzymatic activity in hydrolysis of triglyceride, as compared with a batch experiment. These results demonstrate that the microreactor using mesoporous silica performs not only the reagent-less enzyme immobilization but also the high reactivity of the enzyme.
Journal: Materials Letters - Volume 63, Issue 28, 30 November 2009, Pages 2445–2448