کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1677856 1009917 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Energy-filtered transmission electron microscopy of biological samples on highly transparent carbon nanomembranes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد فناوری نانو (نانو تکنولوژی)
پیش نمایش صفحه اول مقاله
Energy-filtered transmission electron microscopy of biological samples on highly transparent carbon nanomembranes
چکیده انگلیسی

Ultrathin carbon nanomembranes (CNM) comprising crosslinked biphenyl precursors have been tested as support films for energy-filtered transmission electron microscopy (EFTEM) of biological specimens. Due to their high transparency CNM are ideal substrates for electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI) of stained and unstained biological samples. Virtually background-free elemental maps of tobacco mosaic virus (TMV) and ferritin have been obtained from samples supported by ∼1 nm thin CNM. Furthermore, we have tested conductive carbon nanomembranes (cCNM) comprising nanocrystalline graphene, obtained by thermal treatment of CNM, as supports for cryoEM of ice-embedded biological samples. We imaged ice-embedded TMV on cCNM and compared the results with images of ice-embedded TMV on conventional carbon film (CC), thus analyzing the gain in contrast for TMV on cCNM in a quantitative manner. In addition we have developed a method for the preparation of vitrified specimens, suspended over the holes of a conventional holey carbon film, while backed by ultrathin cCNM.

Research highlights
► We examine ultrathin carbon nanomembranes (CNM) as supports for biological TEM.
► CNM comprise crosslinked biphenyl precursors.
► CNM supports enable background-free elemental mapping of heavy and light elements.
► We perform cryoEM of ice-embedded biological samples on graphene-like conductive CNM.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultramicroscopy - Volume 111, Issue 5, April 2011, Pages 342–349
نویسندگان
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