Electrochemical immunosensor for prostate-specific antigen using self-assembled oligophenylethynylenethiol monolayer containing dendrimer

Electrochemical immunosensor for prostate-specific antigen (PSA) was fabricated with a self-assembled 4-(2-(4-(acetylthio)phenyl)ethynyl)benzoic acid (APBA) as a bioreceptor. In order to enhance the electrochemical activity of PSA detection, poly(amidoamine) dendrimer was linked on the APBA self-assembled monolayer (SAM). The formation and electrical properties of the SAMs were investigated by surface plasmon resonance and cyclic voltammetry, respectively. The surface morphology of PSA sandwich complex onto the APBA SAM was studied by atomic force microscopy.