کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1678698 | 1518371 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real-time imaging of DNA–streptavidin complex formation in solution using a high-speed atomic force microscope
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
فناوری نانو (نانو تکنولوژی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Real-time imaging of DNA–streptavidin complex formation in solution using a high-speed atomic force microscope Real-time imaging of DNA–streptavidin complex formation in solution using a high-speed atomic force microscope](/preview/png/1678698.png)
چکیده انگلیسی
The direct observation of individual molecules in action is required for a better understanding of the mechanisms of biological reactions. We used a high-speed atomic force microscope (AFM) in solution to visualize short DNA fragments in motion. The technique represents a new approach in analyzing molecular interactions, and it allowed us to observe real-time images of biotinylated DNA binding to/dissociating from streptavidin protein. Our results show that high-speed AFMs have the potential to reveal the mechanisms of molecular interactions, which cannot be determined by analyzing the average value of mass reactions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultramicroscopy - Volume 107, Issues 2–3, February–March 2007, Pages 184–190
Journal: Ultramicroscopy - Volume 107, Issues 2–3, February–March 2007, Pages 184–190
نویسندگان
Mime Kobayashi, Koji Sumitomo, Keiichi Torimitsu,