کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
168154 1423404 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Three-stage fermentation and kinetic modeling of bioflocculant by Corynebacterium glutamicum
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Three-stage fermentation and kinetic modeling of bioflocculant by Corynebacterium glutamicum
چکیده انگلیسی

Fermentation of bioflocculant with Corynebacterium glutamicum was studied by way of kinetic modeling. Lorentzian modified Logistic model, time-corrected Luedeking–Piret and Luedeking–Piret type models were proposed and applied to describe the cell growth, bioflocculant synthesis and consumption of substrates, with the correlation of initial biomass concentration and initial glucose concentration, respectively. The results showed that these models could well characterize the batch culture process of C. glutamicum at various initial glucose concentrations from 10.0 to 17.5 g·L− 1. The initial biomass concentration could shorten the lag time of cell growth, while the maximum biomass concentration was achieved only at the optimal initial glucose concentration of 16.22 g·L− 1. A novel three-stage fed-batch strategy for bioflocculant production was developed based on the model prediction, in which the lag phase, quick biomass growth and bioflocculant production stages were sequentially proceeded with the adjustment of glucose concentration and dissolved oxygen. Biomass of 2.23 g·L− 1 was obtained and bioflocculant concentration was enhanced to 176.32 mg·L− 1, 18.62% and 403.63% higher than those in the batch process, respectively, indicating an efficient fed-batch culture strategy for bioflocculant production.

A three-stage fed-batch strategy was proposed for bioflocculant production by C. glutamicum. In the three stages, i.e. the lag phase, the quick biomass growth and the bioflocculant production stages, the glucose concentration and dissolved oxygen were adjusted according to the prediction results from the modified kinetic models presented in this study. Results show that the biomass and bioflocculant production are largely improved in this three-stage fermentation over the conventional fed-batch fermentation.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Chemical Engineering - Volume 23, Issue 1, January 2015, Pages 219–226
نویسندگان
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