Adsorption and antibacterial effect of copper-exchanged montmorillonite on Escherichia coli K88

Calcium montmorillonite (Ca-MMT), sodium montmorillonite (Na-MMT) and acid-activated montmorillonite (AAM), and their Cu2+-exchanged montmorillonites (Cu-MMT), Cu*Ca-MMT, Cu*Na-MMT and Cu*AAM, were used to study the antibacterial activity on Escherichia coli K88. AAM, Na-MMT and Ca-MMT showed some ability to reduce bacterial plate counts by 37.4%, 13.4% and 14.2%, respectively. Exchanging the montmorillonite with Cu2+ enhanced the antibacterial activity. The Cu*AAM, Cu*Na-MMT and Cu*Ca-MMT reduced the bacterial plate counts by 98.6%, 97.5% and 95.6%. Attempts were made to study the desorption of Cu2+ by washing with sterile physiological saline solution for 24 h. The washing solutions did not show a significant reduction of the bacterial counts, while the washed Cu-MMT retained their full antibacterial activity. Results from time-depending studies showed that the reduction of the bacterial counts by Cu-MMT increased during 24 h. The ranking of antibacterial activity of the three Cu-MMT was as Cu*AAM > Cu*Na-MMT > Cu*Ca-MMT. E. coli thrived optimally in a pH range from 5 to 7. Beyond this range, the bacterial counts decreased as the pH reduced the viability of the bacteria. The ranking of antibacterial activity of Cu-MMT was not affected by pH. The mechanism by which bacterial counts are reduced may involve the enhanced affinity of Cu-MMT for E. coli K88 and the antibacterial activity of Cu2+.