کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
16971 42627 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of glucose biosensor based on reconstitution of glucose oxidase onto polymeric redox mediator coated pencil graphite electrodes
ترجمه فارسی عنوان
توسعه بیوسنسور گلوکز بر اساس بازسازی گلوکز اکسیداز بر روی الکترودهای گرافیتی مداد با مداد رنگی مجدد پلیمر
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Poly(GMA-co-VFc)/BA/FAD/GOx biosensing electrode was prepared.
• The biosensor seems to be fast to respond and sensitive.
• The reconstitution of enzyme in this study is an effective method to produce reliable biosensors.

In this study, a novel glucose biosensor was fabricated by reconstitutional immobilization of glucose oxidase (GOx) onto a poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)) film coated pencil graphite electrode (PGE). The amperometric current response of poly(GMA-co-VFc)-GOx to glucose is linear in the concentration range between 1 and 16 mM (correlation coefficient of 0.9998) with a detection limit of 2.7 μM (S/N = 3). Experimental parameters were studied in detail and optimized, including the pH and temperature governing the analytical performance of the biosensor. The stability and reusability of the biosensor as well as its kinetic parameters have also been studied.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 68, January 2015, Pages 69–76
نویسندگان
, , ,