Characterization of gamma radiation inducible thioredoxin h from Spirogyra varians

In this study, thioredoxin h (Trxh) was isolated and characterized from the fresh water green alga Spirogyra varians, which was one amongst the pool of proteins induced upon gamma radiation treatment. cDNA clones encoding S. varians thioredoxin h were isolated from a pre-constructed S. varians cDNA library. Trxh had a molecular mass of 13.5 kDa and contained the canonical WCGPC active site. Recombinant Trxh showed the disulfide reduction activity, and exhibited insulin reduction activity. Also, Trxh had higher 5,5′-dithiobis(2-nitrobenzoic acid) reduction activity with Arabidopsis thioredoxin reductase (TR) than with Escherichia coli TR. Specific expression of the Trxh gene was further analyzed at mRNA and protein levels and was found to increase by gamma irradiation upto the absorbed dose of 3 kGy, suggesting that Trxh may have potential functions in protection of biomolecules from gamma irradiation.

► Thioredoxin h (Trxh) was isolated and characterized from alga Spirogyra varians.
► S. varians thioredoxin h (SvTrxh) conserved the canonical WCGPC active site.
► The mRNA and protein levels of SvTrxh were increased by gamma irradiation.
► SvTrxh exhibited insulin reduction activity and a high DTNB reduction activity.
► Radioprotective function was observed in SvTrxh transgenic bacteria.