Characterization of five fresh water microalgae with potential for biodiesel production

• Characterization genetic of five freshwater microalgae strains of the family Scenedesmaceae was executed.
• The microalgae strains under study were coded as SCRE-1, SCRE-2, DSRE-1, DSRE-2 and CORE-1.
• Studies indicated that SCRE-1 and SCRE-2 belong to Scenedesmus, DSRE-1 and DSRE-2 to Desmodesmus, and CORE-1 to Coelastrum.
• Biodiesel properties from fatty acids produced before and after N-limitation were estimated.
• SCRE-2 biodiesel before N-limitation, and DSRE-1 and DSRE-2 biodiesels after limitation showed good quality parameters.

Microalgae biodiesel feedstocks have been investigated by numerous research groups to overcome dependence on fossil fuels. This study describes a detailed characterization of five freshwater microalgae strains of the family Scenedesmaceae, based on cell wall ultrastructure, ITS-2 sequence and secondary structure analysis, as well as the estimation of biodiesel properties from fatty acids produced before and after N-limitation. Characterization permitted the reclassification of SCRE-1 and SCRE-2 strains into the subgenus Scenedesmus; DSRE-1 and DSRE-2 strains into the subgenus Desmodesmus; and CORE-1 strain into the genus Coelastrum. Transesterification of fatty acids of the five strains was performed before and after N-limitation, and seven important biodiesel quality parameters were predicted by applying selected correlations. These parameters were then compared to the corresponding specifications in the American and European biodiesel standards. N-limitation promoted higher yields of biomass (up to 3.5 times) and lipids (up to 4.1 times) in all strains under study. Moreover, it was found that SCRE-2 was the only biodiesel that met all estimated parameters of the more stringent European standard before N-limitation. This was also true for DSRE-1 and DSRE-2 biodiesels after limitation, with the exception of their oxidative stability parameter, whose values met the limit of the American but not the European standard.