کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
17422 42667 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Availability of tyrosine amide for α-chymotrypsin-catalyzed synthesis of oligo-tyrosine peptides
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Availability of tyrosine amide for α-chymotrypsin-catalyzed synthesis of oligo-tyrosine peptides
چکیده انگلیسی

Oligo-tyrosine peptides such as Tyr-Tyr having angiotensin I-converting enzyme (ACE) inhibitory activity could be synthesized by α-chymotrypsin-catalyzed reaction with l-tyrosine ethyl ester in aqueous media. However, peptide yield in the reaction was below 10%. Since l-tyrosine amide showed highly nucleophilic activity for the deacylation of enzyme through which a new peptide bond was made, its application to the enzymatic peptide synthesis was evaluated in this study. Addition of tyrosine amide into the reaction produced Tyr-Tyr-NH2, of which yield exceeded 130% on the basis of tyrosine ethyl ester. Although purified Tyr-Tyr-NH2 did not inhibit ACE activity, α-chymotrypsin could act on the dipeptide amide and convert about 40% of it to Tyr-Tyr. The use of both ester and amide forms of tyrosine is expected to be a potent procedure for α-chymotrypsin-catalyzed synthesis of antihypertensive peptides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 45, Issues 6–7, December 2009, Pages 457–462
نویسندگان
, , , ,