In vivo fluorescence imaging for Cu2+ in live mice by a new NIR fluorescent sensor

• A NIR sensor was developed to display the Cu2+-specific emission quenching effect.
• The in vivo fluorescent Cu2+ imaging in live mice was realized for the first time.
• The large Stokes shift reduces the sensor self-absorption, favoring in vivo imaging.
• The sensor displays the minor photobleaching interference in imaging.
• The intramolecular CT effect in sensor is essential for its in vivo imaging behavior.

Modifying the BODIPY fluorophore with a 4-aminostyryl group forms a NIR fluorescent Cu2+ sensor with a large Stokes shift, high photostability and a high quantum yield. The in vivo Cu2+ fluorescence imaging in live mice was realized for the first time with this sensor, profiting from its reduced self-absorption and minor photobleaching.

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