کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1764824 | 1020073 | 2013 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness](/preview/png/1764824.png)
We have explored the role of Ca2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca2+]i), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca2+]i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca2+]i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca2+ influx through stretch-activated channels, then results in microfilament disruption.
► Simulated weightlessness disrupts microfilaments in osteoblasts.
► Simulated weightlessness inhibits the osteoblastic proliferation.
► Simulated weightlessness inhibits the migration of osteoblasts.
► Simulated weightlessness induces Ca2+ influx through stretch-activated channels.
► Ca2+ influx causes microfilament disruption induced by simulated weightlessness.
Journal: Advances in Space Research - Volume 51, Issue 11, 1 June 2013, Pages 2058–2068