Clean-up and concentration of manganese peroxidases recovered during the biodegradation of Eucalyptus grandis by Ceriporiopsis subvermispora

Ceriporiopsis subvermispora was utilized for the biodegradation of Eucalyptus grandis in the presence or absence of co-substrates (glucose and corn steep liquor) during 7, 14 and 28 days. The biodegraded chips were used to prepare enzymatic extracts that, after pre-treatment with activated charcoal, were enriched in activity of manganese peroxidases (MnPs) with anion-exchange chromatography followed by elution with NaCl. Samples of these extracts were then analyzed by denaturing electrophoresis in order to compare the profiles of proteins and MnPs. Depending on the biodegradation period and on the presence or absence of co-substrates in the culture, one or two major proteins (relative molecular weights of 46.8 ± 0.6 and 51.6 ± 1.0 kDa) were identified. The presence of MnPs activity was further confirmed by native electrophoresis followed by phenol red staining.