کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1787556 | 1023445 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A novel thin film amperometric urea biosensor based on urease-immobilized on poly(N-glycidylpyrrole-co-pyrrole) A novel thin film amperometric urea biosensor based on urease-immobilized on poly(N-glycidylpyrrole-co-pyrrole)](/preview/png/1787556.png)
An amperometric biosensor has been developed for the measurement of urea. To construct the proposed biosensor urease enzyme was immobilized onto poly(N-glycidylpyrrole-co-pyrrole) conducting film by direct covalent attachment. The biosensor surface was characterized by FTIR spectroscopy and atomic force microscopy (AFM). The response studies were carried out as a function of urea concentration with amperometric measurements. The biosensor showed a linear current response to the urea concentration ranging from 0.1 to 0.7 mM. The urea biosensor exhibited a sensitivity of 4.5 μA/mM with a response time of 4 s. The factors influencing on the performance of the resulting biosensor were also studied in detail.
► Poly(N-glycidylpyrrole-co-pyrrole) film enable direct covalent immobilization without any reagent.
► The biosensor seems to be simple to prepare, fast to respond, inexpensive and sensitive.
► The conducting polymer film used in this study is an effective platform to produce reliable biosensors.
Journal: Current Applied Physics - Volume 11, Issue 4, July 2011, Pages 1083–1088