کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1793028 1023663 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Physicochemical properties of artificial proteins that accelerate nucleation of crystalline calcium phosphate
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم فیزیک ماده چگال
پیش نمایش صفحه اول مقاله
Physicochemical properties of artificial proteins that accelerate nucleation of crystalline calcium phosphate
چکیده انگلیسی

We have created 18 artificial proteins by embedding two calcification motifs found in a protein involved in bone and dentin formation. The relationship between the physicochemical properties of the proteins and their in vitro mineralization activities was investigated. Two slightly acidic proteins nucleated octacalcium phosphate (OCP) at a low protein concentration from a solution where precipitation did not occur without the proteins. Among 16 basic proteins, only one protein nucleated OCP at the same protein concentration. At a higher protein concentration, four proteins self-assembled to become large aggregates, and these four proteins nucleated OCP. Among 14 proteins without the self-assembling property, only five proteins nucleated OCP. None of the proteins formed α-helix or ß-sheet structures. These results suggest that the proteins that had the self-assembling property appeared to exhibit nucleation activity with high frequency and that the formation of the ordered backbone conformation was not required for proteins to promote nucleation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Crystal Growth - Volume 314, Issue 1, 1 January 2011, Pages 190–195
نویسندگان
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