کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
18037 | 42710 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of dextransucrase on regenerated benzoyl cellulose carriers
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immobilization of dextransucrase on regenerated benzoyl cellulose carriers Immobilization of dextransucrase on regenerated benzoyl cellulose carriers](/preview/png/18037.png)
چکیده انگلیسی
The regeneration of the benzoyl cellulose carrier was accomplished by treatment by Triton X-100, sodium dodecyl sulfate (SDS), or alkyldimethylbenzyl-ammonium chloride (ADMB) detergent solutions, or by NH4SCN as chaotropic agent. It was found that Triton X-100, as a more hydrophobic substance, was linked to the carrier, eluting the protein from the carrier. Regenerated in this way, the carrier very efficiently binds new amounts of proteins, including dextransucrase, without regarding the fact that Triton X-100 was washed out by alcohol, or remained linked to the carrier. Dextransucrase immobilization at this regenerated carriers yielded high values for the immobilized activity, total preserved activity and immobilization efficiency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 763–768
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 763–768
نویسندگان
Živomir Petronijević, Suzana Ristić, Dragan Pešić, Andrija Šmelcerović,