کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
18179 | 42713 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Asymmetric reduction of 2-octanone in water/organic solvent biphasic system with Baker's yeast FD-12 Asymmetric reduction of 2-octanone in water/organic solvent biphasic system with Baker's yeast FD-12](/preview/png/18179.png)
In the aqueous medium the asymmetric reduction of 2-octanone with Baker's yeast FD-12 was inhibited severely by the substrate and the product at higher concentrations, which resulted in a low yield and a low e.e. value of the object product (S)-2-octanol. Seven water/organic solvent biphasic systems were assayed in this work. FD-12 showed the best tolerance, catalytic activity and enantioselectivity in the water/n-dodecane system among the biphasic systems assayed. The metabolic activity retention (MAR) and viability of FD-12 in the n-dodecane containing system were 98% and 91.6%, respectively. The influence of the volume ratio Vaq/Vorg, temperature, pH value and the substrate concentration on the yield of 2-octanol and the e.e.(S) value was investigated. Under the optimum conditions (in the water/n-dodecane biphasic system, Vaq/Vorg 20/5, pH 8.0, 32 °C, 2-octanone concentration 500 mM), the final concentration of 2-octanol and the e.e.(S) value reached 105.60 mM and 89.38%, respectively, after 96 h reduction. The water/n-dodecane biphasic system with FD-12 was advantageous for the production of (S)-2-octanol of high final concentration and high e.e.(S) value.
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1305–1311