کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
181872 459412 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A disposable impedance sensor for electrochemical study and monitoring of adhesion and proliferation of K562 leukaemia cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A disposable impedance sensor for electrochemical study and monitoring of adhesion and proliferation of K562 leukaemia cells
چکیده انگلیسی

A polyaniline-modified screen-printed carbon electrode (PANI/SPCE) was prepared by electropolymerization for the construction of a novel disposable cell impedance sensor. The conductive polymer improved greatly the electron transfer of SPCE and was very effective for cell immobilization. The adhesion of cells increased the electron transfer resistance (Ret) of redox probe on the PANI/SPCE surface, producing an impedance sensor for K562 leukaemia cells with a semilogarithm linear range from 104 to 107 cells ml−1 and a limit of detection of 8.32 × 103 cells ml−1 at 10σ. The proliferation of cells on the conductive polymer increased the Ret, leading to a novel way to monitor the growth process of cells on the PANI/SPCE. The electrochemical monitoring indicated K562 leukaemia cells cultured in vitro on the PANI surface were viable for 60 h, consistent with the analysis from microscopic imaging and MTT assay. This method for monitoring the surface proliferation and detecting the number of viable cells was simple, low-cost and disposable, thus providing a convenient avenue for electrochemical study of cell immobilization, adhesion, proliferation and apoptosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 9, Issue 5, May 2007, Pages 953–958
نویسندگان
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