کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18211 42714 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4-lacZ-based cadmium and copper biosensor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4-lacZ-based cadmium and copper biosensor
چکیده انگلیسی

Based on the effects of cadmium and copper on KlHIS4 transcription when expressed in either Kluyveromyces lactis or Saccharomyces cerevisiae, a yeast biosensor is presented. It consists of a lacZ fused to a KlHIS4 447 bp long promoter expressed in K. lactis, and as control, a second fusion with a shorter promoter not regulated by metals. This eukaryotic system can be used to detect cadmium and copper concentrations of 0.3–3 mM (or higher) within minutes using X-GAL filter assay and other variants of β-galactosidase assays developed in multititer wells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 40, Issue 1, 6 December 2006, Pages 67–70
نویسندگان
, ,