کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
18366 | 42720 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Screening methods for synthetic activity of lipases
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
In order to optimize synthetic biocatalytic transformations catalyzed by lipases, a large number of enzyme preparations must be tested. Moreover, to design an enzyme for an industrial application it is generally necessary to realize enzyme evolution to improve enzyme performances, for instance thermostability, activity or selectivity. The crucial step is then the screening of the optimal catalyst. Because hydrolytic and synthetic activities are not always correlated, specific screening methods for synthesis activity of lipases are needed. In this work, we report three sensitive and specific screening methods to measure synthetic activity of lipases in vivo on an agar plate and in vitro, directly from culture broth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 390–393
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 390–393
نویسندگان
Georgina Sandoval, Alain Marty,