کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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18391 | 42721 | 2006 | 4 صفحه PDF | دانلود رایگان |
Lipase constitutes an interesting class of enzyme with many biotechnological applications. However, the development of a fruitful process must be set up to obtained a product compatible with the industrial and commercial needs. Here, we report the development of such a process for the extracellular lipase secreted by the yeast Yarrowia lipolytica. The enzyme production, carried out in a 2000 L bioreactor, led to a lipase activity of approximately 1100 U mL−1 after 53 h of fermentation. The post-culture treatment, consisting of a centrifugation, a filtration and an ultra-filtration steps, led to 15-fold volume reduction and a 8-fold increased of the lipase activity. Finally, addition of 12% of milk powder and 3% of gum arabic before spray-drying dehydration led to a stable powder with a lipolytic activity of 37,500 U g D.W.−1.
Journal: Enzyme and Microbial Technology - Volume 38, Issue 6, 1 April 2006, Pages 756–759