کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
18458 | 42724 | 2006 | 6 صفحه PDF | دانلود رایگان |
High-performance liquid chromatography analysis of crude chloroform and methanol extracts as well as methanol preparations subjected to enzymatic hydrolysis indicated two-fold higher accumulation of umbelliferone in transformed Ammi majus L. callus grown on medium exposed to ADR-4® (energy stimulator plate) in comparison to the control cultures. Elicitation with ADR-4® induced also two times higher accumulation of bergapten. Three different techniques: Soxhlet extraction, ultrasonication and accelerated solvent extraction (ASE) applied for the extraction of coumarins, furanocoumarins and corresponding glycosides from transformed callus of A. majus elicited by ADR-4® plates were compared. ASE is shown to be the most efficient extraction method.
Journal: Enzyme and Microbial Technology - Volume 39, Issue 7, 3 November 2006, Pages 1386–1391