کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
18492 | 42725 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of glucose-6-phosphate dehydrogenase releasing from Candida guilliermondii by disruption with glass beads
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The release of glucose-6-phosphate dehydrogenase (G6PD) from Candida guilliermondii, grown on rice straw hydrolysate, by vortex agitation with glass beads was studied. The effects of cell suspension concentration and disruption time on volumetric activity, total protein and specific activity were evaluated from a 22 full-factorial central composite design. By using the response surface methodology, a second-order model was proposed to represent enzyme specific activity as a function of disruption time (X1) and cell suspension concentration (X2). Under optimum conditions (X1 = 5 min and X2 = 11.5 g l−1 the model predicted a G6PD specific activity of 0.88 U mg−1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 591–595
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 591–595
نویسندگان
Daniela B. Gurpilhares, Francislene A. Hasmann, Adalberto Pessoa Jr., Inês C. Roberto,