کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18543 42725 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic resolution of (R,S)-ethyl 2-chloromandelate in biphasic media using hydrolase of Klebsiella oxytoca
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Kinetic resolution of (R,S)-ethyl 2-chloromandelate in biphasic media using hydrolase of Klebsiella oxytoca
چکیده انگلیسی

For the first time, the hydrolytic resolution of (R,S)-ethyl 2-chloromandelate in biphasic media by using hydrolase of Klebsiella oxytoca as a promising biocatalyst is reported for producing the desired (R)-ethyl 2-chloromandelate as an important intermediate for the synthesis of Clopidogrel. Effects of various operating parameters on the enzyme activity and enantioselectivity were systematically studied, from which the optimal condition of using isooctane as the organic phase, enzyme concentration of 2 mg/ml, temperature of 55 °C, and pH 6 of the aqueous phase was selected. The kinetic constants k2R = 4.6 × 10−3 mmol/h g, k2S = 18.2 mmol/h g, kmR = 0.72 mM, kmS = 0.90 mM, and KI = 65.5 mM were further estimated from the kinetic analysis at the optimal condition. By considering the acid product inhibition and non-enzymatic hydrolysis for the enzymatic resolution, good agreements of the time-course conversions from experiments and the theoretical prediction were also obtained.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 930–935
نویسندگان
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