کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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18724 | 42738 | 2007 | 6 صفحه PDF | دانلود رایگان |
The industrial process of a whole-cell microbial transformation is usually hindered by the toxicity or inhibition of a high substrate/product concentration. Especially in a biocatalytic redox process, the cofactor regeneration with the viable cells is a key of the whole cell microbial transformation. In present work, growing culture of Saccharomyces cerevisiae (baker's yeast) in a nonionic surfactant mediated cloud point system was carried out. Then the tolerances of baker's yeast cells against a high substrate/product concentration and a long term of biocatalytic reduction of acetophenone to produce 1-phenylethanol as a model were examined in the cloud point system. The long term of cofactor regeneration at a high substrate concentration in the cloud point system make the cloud point system potential for further development as a novel two-phase partitioning system for production of chiral compounds by microbial transformation.
Journal: Enzyme and Microbial Technology - Volume 41, Issue 3, 2 August 2007, Pages 296–301