Real-time immunohistochemistry analysis of embedded tissue

We present a novel analysis of membrane–protein expression in tissue sections based on semi-automatic real-time measurement using LigandTracer® technology. A commercial antiHER2 antibody developed for immunohistochemistry used in this setup was revealed to have sub-optimal interaction with tissue when analyzed as recommended for immunohistochemistry. We therefore think that real-time measurement of tissue, offering direct and quantitative membrane–protein interaction analysis, can lead to improved reproducibility and eliminate the subjective operator dependences that classical immunohistochemsitry suffers from.