Biologically active equine estrogens and sulfate conjugates labelled with tritium at high specific activity

Methods are presented to tritiate and characterize the steroid equilin as well as a structurally related 17-alpha alcohol metabolite and several 3-position sulfate conjugates.

► This paper describes the synthesis of [3H] equilin and a metabolite.
► Iodination—catalytic tritium dehalogenation was employed.
► This paper also describes the synthesis of several tritiated sulfate conjugates.