کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1882617 1043241 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Papain incorporated chitin dressings for wound debridement sterilized by gamma radiation
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
پیش نمایش صفحه اول مقاله
Papain incorporated chitin dressings for wound debridement sterilized by gamma radiation
چکیده انگلیسی

Wound debridement is essential for the removal of necrotic or nonviable tissue from the wound surface to create an environment conducive to healing. Nonsurgical enzymatic debridement is an attractive method due to its effectiveness and ease of use. Papain is a proteolytic enzyme derived from the fruit of Carica papaya and is capable of breaking down a variety of necrotic tissue substrates. The present study was focused on the use of gamma radiation for sterilization of papain dressing with wound debriding activity. Membranes with papain were prepared using 0.5% chitin in lithium chloride/dimethylacetamide solvent and sterilized by gamma radiation. Fluid absorption capacity of chitin–papain membranes without glycerol was 14.30±6.57% in 6 h. Incorporation of glycerol resulted in significant (p<0.001) increase in the absorption capacity. Moisture vapour transmission rate of the membranes was 4285.77±455.61 g/m2/24 h at 24 h. Gamma irradiation at 25 kGy was found suitable for sterilization of the dressings. Infrared (IR) spectral scanning has shown that papain was stable on gamma irradiation at 25–35 kGy. The irradiated chitin–papain membranes were impermeable to different bacterial strains and also exhibited strong bactericidal action against both Gram-positive and Gram-negative bacteria. The fluid handling characteristics and the antimicrobial properties of chitin–papain membranes sterilized by gamma radiation were found suitable for use as wound dressing with debriding activity.

Highlight
► Use of gamma radiation for sterilization of papain wound dressing was studied.
► Fluid handling and antimicrobial properties of irradiated dressings was evaluated.
► Gamma irradiation at 25 kGy was found suitable for sterilization of the dressings.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Radiation Physics and Chemistry - Volume 81, Issue 11, November 2012, Pages 1781–1785
نویسندگان
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