کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1886481 1043527 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Radiation synthesis of seroalbumin nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
پیش نمایش صفحه اول مقاله
Radiation synthesis of seroalbumin nanoparticles
چکیده انگلیسی

Synthesis of small polymeric particles can be achieved by ionizing radiation technology via intramolecular crosslinking by gamma rays onto soluble polymer molecules in random coil conformation. Differently soluble globular proteins are naturally densely packed structures. Fragmentation and aggregation processes have been reported for irradiated globular proteins solutions with ionizing radiations.In this work we describe protein-based nanoparticles prepared by gamma irradiation of a soluble and globular protein, such as seroalbumin, as the basic building blocks keeping its original conformational shape. Protein nanoparticles in the range 20–40 nm were detected after gamma irradiation of the aqueous protein solution in the presence of polar organic solvents. Nanoparticles were characterized by DLS, fluorescence, and UV and CD spectroscopy, showing that the protein molecules keep their general three-dimensional structure into the created nanoparticle.


► Protein nanoparticles were prepared by gamma irradiation of a solution of a globular protein.
► Albumin nanoparticles were achieved in the presence of polar organic solvents.
► Protein nanoparticles are in the range of 20 to 40 nm.
► Nanoparticles were characterized by DLS, fluorescence, and UV and CD spectroscopy.
► Protein molecules keep their general three-dimensional structure into the created nanoparticle.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Radiation Physics and Chemistry - Volume 81, Issue 9, September 2012, Pages 1417–1421
نویسندگان
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