کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1886529 1043529 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of gamma ray-induced DNA damage in Lasioderma serricorne using the comet assay
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
پیش نمایش صفحه اول مقاله
Assessment of gamma ray-induced DNA damage in Lasioderma serricorne using the comet assay
چکیده انگلیسی

We attempted a DNA comet assay under alkaline conditions to verify the irradiation treatment of pests. Lasioderma serricorne (Fabricius) were chosen as test insects and irradiated with gamma rays from a 60Co source at 1 kGy. We conducted the comet assay immediately after irradiation and over time for 7 day. Severe DNA fragmentation in L. serricorne cells was observed just after irradiation and the damage was repaired during the post-irradiation period in a time-dependent manner. The parameters of the comet image analysis were calculated, and the degree of DNA damage and repair were evaluated. Values for the Ratio (a percentage determined by fluorescence in the damaged area to overall luminance, including intact DNA and the damaged area of a comet image) of individual cells showed that no cells in the irradiated group were included in the Ratio<0.1 category, the lowest grade. This finding was observed consistently throughout the 7-day post-irradiation period. We suggest that the Ratio values of individual cells can be used as an index of irradiation history and conclude that the DNA comet assay under alkaline conditions, combined with comet image analysis, can be used to identify irradiation history.


► We investigated the DNA comet assay to verify the irradiation of pests.
► Ratio and Tail Moment were higher in irradiated groups than in the control group.
► The DNA comet assay can be used to identify irradiation history.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Radiation Physics and Chemistry - Volume 81, Issue 3, March 2012, Pages 316–321
نویسندگان
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