Effect of the γ-radiation on phenol fractions obtained from the leaves of Echinodorus macrophyllus Mich.

Echinodorus macrophyllus Mich. (Chapéu-de-couro) is popularly used as diuretic, anti-arrhythmic, anti-inflammatory, and anti-rheumatic agents. Leaves of this species are largely commercialized and show high level of microbiological contamination (bacteria and fungi). This work describes the effect of the 60Co γ-radiation on the phenol fractions obtained from the leaves of E. macrophyllus. trans-Ferulic acid, (E)-caffeoyltartronic acid, 6-C-(1-hexitol)-apigenin, and 6-C-(1-hexitol)-luteolin were isolated by preparative HPLC. HPLC chromatograms showed concentration changes of some phenolic constituents, suggesting the formation of radiolytic products. The phenol fractions were active against Bacillus subitilis and Staphylococcus aureus and showed high antioxidant activity. However, the antibacterial and antioxidant activities reduced when the absorbed dose was increased.

► Effects of the γ-radiation on the chemical constituents of the leaves of E. macrophyllus.
► To determine the conditions, which are sufficient for microbiological decontamination.
► Analysis by HPLC of the phenol fractions obtained from the leaves of E. macrophyllys.
► Effects of the γ-radiation in the antioxidant and antimicrobial activities of E. macrophyllus.