Extraction, purification and application of thermostable and halostable alkaline protease from Bacillus alveayuensis CAS 5 using marine wastes

• Study proposed on utilization of marine waste for inexpensive protease production.
• The protease from this study had higher temperature, pH and detergents stability.
• Application in stain removal could be useful in textile industries.
• Considering production cost and reclamation of waste seems to be promising approach.

A marine bacterium Bacillus alveayuensis CAS 5 produced protease when grown at 55 °C for 60 h in 100 ml of basal medium containing 1% SSP (w/v) and purified to 7.77 fold with specific activity of 518.78 U/mg. The optimum temperature, pH and NaCl for enzyme activity were 50 °C, 9 and 35% respectively. The enzyme was highly stable even at 80 °C, pH 12, 35% NaCl and presence of ionic, non-ionic and commercial detergents. The protease was investigated for its application as cleansing additive in blood stain removal. The preset study emphasized that marine wastes can be utilized to generate high value-added products and hidden potential in the production of functional foods.